Morphological and Molecular Analysis of Genetic Diversity among Some ‘Sukkary’ Mango (Mangifera indica L.) Genotypes
The present study aimed to investigates the morphological characterization and estimate genetic polymorphism and relationships among five mango accessions (collected from different governorates) based on ISSR markers. Seventy-eight morphological characteristics were studied to describe the fruit and stone. The collected mango accessions exhibited a wide range of differences in fruit and stone characteristics. All the studied accessions had a roundish fruit shape smooth and waxy yellow skin, soft pulp texture and obtuse shape of fruit apex. Differences among accessions were observed in fruit ground color, fruit beak type, pulp aroma. Also the quantitative characteristics of fruit and seed showed differences between the collected accessions. Inter-simple sequence repeats (ISSR) markers were used to study the genetic diversity and phylogenetic relationships among the collected accessions. The twelve ISSR primers produced a total number of amplified bands ranged from 6 to 19 fragments. The highest number of fragments was 19 bands for (TC)8GT primer. While, (CA)6GT primer generated the lowest number of amplicons (6 bands). The average number of fragments/primer was (11.25) and the size of these fragment ranged from 326-3125 bps. The percentage of polymorphism revealed by the different primers ranged from 7.14 to 66.67 % with average of 42.86%.