Total Phenolic Content and In Vitro Antioxidant Activity of Vigna sinensis

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Vigna sinensis also known as long-podded cowpea or Chinese long bean (Family:Fabaceae) is most widely grown in Southeast Asia. They are a good source of protein, vitamin A, vitamin C, iron, phosphorus, and potassium. The antioxidant potential of crude methanol extract, chloroform, and ethyl acetate soluble fractions of Vigna sinensis was screened for in- vitro antioxidant activity using total phenolic content, ferric reducing power, 2,2’-azinobis-(3-ethylbenzothiazoline-6- sulphonic acid) (ABTS) assay, ferric thiocyanate (FTC) and thiobarbituric acid (TBA) tests. It was found that ethyl acetate fraction have maximum amount of polyphenolics compounds (2.69 mg/g GAE in concentration 0.5 mg/mL); more effective than methanol and chloroform extract.This fraction also exhibited fairly good antioxidant activity with in both TBA (17.39% mg/g GAE) and FTC (12.65% mg/g GAE) methods. 

Authors: 
S. Emynur Shafekh
Authors: 
R Mohd Adzim Khalili
Authors: 
C.C.W. Catherine
Authors: 
Z.A. Siti Syakiroh
Authors: 
A. Ummu Habibah
Authors: 
A.H. Norhayati
Authors: 
M.Y. Nor Farhanah
Authors: 
Z. Noor Husna
Authors: 
M.B. Siti Nafizah
Authors: 
M. Azlina
Authors: 
A.R. Noor Shahhida Sazura
Authors: 
A.L. Ahmad Zubaidi
Publisher: 
International Food Research Journal
Year: 
2012