Toxicity of ozone gas to conidia of Penicillium digitatum, Penicillium italicum, and Botrytis cinerea and control of gray mold on table grapes

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Penicillium digitatum, Penicillium italicum, and Botrytis cinerea attack fresh fruit and cause significant postharvest decay losses. The toxicity of ozone (O3) gas at different relative humidities to control their conidia was determined. Conidia distributed on cover glasses were exposed to an atmosphere containing 200–350µLL−1 of O3 gas at 35%, 75%, and 95% relative humidity (RH) at 25 °C. O3 gas was produced by UV light generators and passed through three 500mL solutions of saturated MgCl2 (35% RH), NaCl (75% RH), or K2SO4 (95% RH). O3 and RH inside the chamber were monitored. O3 exposures were quantified as concentration x time products adjusted to 1 h (µLL−1 ×h). After exposure to O3 for varying periods, the conidia were removed from the chamber, placed on potato dextrose agar and their germination observed. Conidia died more rapidly at higher humidity than at lower humidity, and P. digitatum and P. italicum were more resistant to O3 than B. cinerea. At 95% RH, 99% of the conidia of P. digitatum, P. italicum, and B. cinerea were incapable of germination after O3 exposures of 817, 732, and 702µLL−1 x h, respectively. At 75% RH, similar inhibition required exposures of 1781, 1274, and 1262µLL−1 x h, respectively. At 35% RH, O3 toxicity declined markedly, and 99% mortality required 11,410, 10,775, and 7713µLL−1 x h, respectively. These values can be used to select O3 gas exposures needed to control these conidia. Conidia of B. cinerea were sprayed on to the surface of table grapes and 2 h later the grapes were exposed to 800–2000µLL−1 x h of O3. O3 at 800µLL−1 x h or more reduced the incidence of infected berries by 85% and 45% on ‘Autumn Seedless’ and ‘Scarlet Royal’ grapes, respectively. Fumigation with O3 can control postharvest pathogenic fungi on commodities that tolerate this gas, or it can be applied to disinfect processing equipment and storage rooms when the produce is not present.

Authors: 
Ragip Ozkan
Authors: 
Joseph L. Smilanick
Authors: 
Ozgur Akgun Karabulut
Publisher: 
Postharvest Biology and Technology
Year: 
2011