In vivo Anti-Diabetic Activity of the Methanolic and Aqueous Bark Extracts of the Plant Emblica officinalis Gaertn
The present studies emphasize the investigation on methanolic and aqueous extract of Emblica officinalis Gaertn. (Family-Euphorbiaceae) stem barks or its anti-diabetic activity in animal model. Alloxan induced diabetes model was used for the study. The standardized doses of 250, 500, 1000 and 500 mg kg-1 body weight of the extract were administered orally to normal and diabetic rats in order to define its hypo-glycemic potential. The effective dose (ED50) was determined by the 1/10th of maximum dose of methanolic thand aqueous stem bark extract of Emblica officinalis respectively. Results depicted that the maximum fall of blood glucose level of normal rats were observed after 6 hour during fasting blood glucose studies, with the dose of 250 mg kg-1 identified as the most effective dose. However, the dose of 250 mg kg-1 of methanolic and aqueous stem barks extract in moderate and mild-diabetic animals produced a maximum fall blood glucose level of 45 and 44% (p < 0.01), respectively. The effect of bark extracts on serum lipid profile (cholesterol, triglycerides, high-density lipoprotein and low-density lipoprotein) were measured in diabetic rats. This evidence clearly indicates that the aqueous and methanolic extract of E. officinalis stem barks have significant hypoglycemic potential as well as anti-diabetic activity.